Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Anti-aging activities of Pyrus pyrifolia var culta plant callus extract

Hyun Jung Kim, Jin Woo Park

College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Muan-gun, Jeonnam, Republic of Korea;

For correspondence:- Jin Park Email: jwpark@mokpo.ac.kr Tel:+82614502704

Received: 16 March 2017 Accepted: 20 June 2017 Published: 31 July 2017

Citation: Kim HJ, Park JW. Anti-aging activities of Pyrus pyrifolia var culta plant callus extract. Trop J Pharm Res 2017; 16(7):1579-1588 doi: 10.4314/tjpr.v16i7.16

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the anti-aging properties of callus extract from Pyrus pyrifolia var. culta with skin regeneration and lightening activities.

Methods: Callus extract of P. pyrifolia var. culta was produced on Murashige and Skoog (MS) medium supplemented with picloram, followed by extraction of the callus biomass in phosphate-buffered saline. The anti-oxidant activity of the extract was assessed using the DPPH free radical-scavenging assay. Inhibition of skin pigmentation by callus extract was evaluated by measuring the melanogenesis of melanoma cells. The skin-regenerating efficacy of the extract was evaluated in terms of its ability to promote procollagen synthesis and fibroblast cell proliferation.

Results: The callus extract, at 10 mg/mL, exhibited 78.7 % free radical-scavenging activity, equivalent to that exhibited by 500 µM ascorbic acid. The 1 mg/mL extract solution afforded a 1.4-fold greater reduction in melanocyte melanin than did a 1 mg/mL arbutin solution. Fibroblast cells treated with 5 mg/mL callus extract exhibited 127 % higher levels of proliferation and 2.0-fold higher levels of procollagen type I C-peptide synthesis, respectively, compared with the untreated controls.

Conclusion: These findings suggest that the callus extract of P. pyrifolia var. culta may be useful as a source of anti-aging cosmetic components with skin lightening and regenerating activities.

Keywords: P. pyrifolia var. culta, Anti-aging, Callus extract, Skin lightening, Anti-tyrosinase activity, Melanogenesis, Wound recovery, Procollagen type I C-pe